5-Amino-1MQ

About 5-Amino-1MQ

5-Amino-1MQ is a small-molecule, cell-permeable, selective inhibitor of nicotinamide N-methyltransferase (NNMT). It is not a peptide. Researchers investigate it in adipocyte models for its effects on cellular SAM/methylation balance, NAD⁺ salvage signalling and lipid storage. Supplied as a lyophilised solid for in vitro laboratory research only.

Research Applications

• Investigated as a selective NNMT inhibitor in enzyme-kinetic and biochemical assays
• Studied for effects on intracellular SAM, MNA and NAD⁺ levels in adipocyte models
• Researched for influence on lipid storage and adipocyte differentiation in vitro
• Associated with modulation of energy-expenditure pathways in murine metabolic models
• Used in research on muscle stem-cell quiescence and skeletal-muscle signalling
• Investigated in oncology research for NNMT-overexpressing tumour-cell lines

Research Mechanism — 5-Amino-1MQ

A simplified summary of what 5-Amino-1MQ is studied for in research models. For laboratory in-vitro use only.

1. 1

   Molecular target

   5-Amino-1MQ has been studied as a competitive inhibitor of NNMT, the enzyme that methylates nicotinamide using S-adenosyl methionine as a methyl donor. Investigations have characterised its binding within the NNMT active site.

   • Binds the NNMT catalytic pocket as a substrate mimetic
   • Competes with nicotinamide for enzyme occupancy
   • Reduces formation of 1-methylnicotinamide (MNA) in vitro
2. 2

   Investigated pathways

   Preclinical studies have explored downstream effects on cellular NAD+ and SAM pools when NNMT activity is suppressed. Associated signalling involves sirtuin and PARP-dependent processes.

   • Elevation of intracellular NAD+ and SAM in adipocyte models
   • Modulation of SIRT1 deacetylase activity
   • Altered methyl-donor flux affecting histone methylation
3. 3

   Preclinical research applications

   Preclinical studies have explored 5-Amino-1MQ in rodent models of diet-induced obesity and in cultured adipocytes. Not approved for human use.

   • Murine high-fat-diet adiposity models
   • 3T3-L1 adipocyte differentiation assays
   • C2C12 myotube studies of mitochondrial output

For research use only. 5-Amino-1MQ is not approved as a medicine in the UK and is not intended for human or veterinary administration. See the compliance page for full terms.